CHEMICAL TOOLS FOR STUDY OF CELL DEATH AND HIGH-THROUGHPUT SCREENING 

We have a long-standing interest in developing novel tools for the study of cell death; for example, we have recently disclosed a small molecule that induces apoptotic cell death with unparalleled speed.   In addition, we have developed high-end technologies for HTS, with an emphasis on methods that can directly report on compound binding (versus traditional assays that measure enzymatic activity). In this vein we work closely with Professor Brian Cunningham at UIUC and have developed novel photonic crystal methods for the rapid detection of inhibitors of protein-DNA interactions, protein-protein interactions, and for direct detection of small molecules binding to proteins.

 

Related Publications:

138.

Monitoring poly(ADP-ribosyl)glycohydrolase activity with a continuous fluorescent substrate

Drown, B. S.; Shirai, T.; Rack, J.G.M.; Ahel, I.; Hergenrother, P. J.

Cell Chem. Biol. 201825, 1562-1570

Link to journal

Article PDF   

116.    

A Small Molecule that Induces Intrinsic Pathway Apoptosis with Unparalleled Speed

Palchaudhuri, R.; Lambrecht, M. J.; Botham, R. C.; Partlow, K. C.; Van Ham, T. J.; Putt, K. S.; Nguyen, L. T.; Kim, S.-H.; Peterson, R. T.; Fan, T. M.; Hergenrother, P. J. 

Cell Reports 2015, 13, 2027-2036.

 

Link to journal

Article PDF

108. 

Detection of Protein-Small Molecule Binding Using a Self-Referencing External Cavity Laser Biosensor​

Zhang, M.; Peh, J.; Hergenrother, P. J.; Cunningham, B. T.

J. Am. Chem. Soc. 2014136, 5840-5843.

 

Link to journal

Article PDF

94. 

External Cavity Laser Biosensor

Ge, C.; Lu, M.; George, S.; Flood, T. A.; Wagner, C.; Zheng, J.; Pokhriyal, A.; Eden, J. G.; Hergenrother, P. J.; Cunningham, B. T.

Lab Chip 201313, 1247-1256.

 

Link to journal

Article PDF

 

UIUC News Highlight

85.

Whole Cell Microtubule Analysis by Flow Cytometry

Morrison, K.C.; Hergenrother, P. J.  

Anal. Biochem. 2012420, 26-32. 

 

Link to journal

Article PDF

78. 

Transcript Profiling and RNA Interference as Tools to Identify Small Molecule Mechanisms and Therapeutic Potential

Palchaudhuri, R.; Hergenrother, P. J.  

ACS Chem. Biol. 20116, 21-33. 

 

Link to journal

Article PDF

76.

High-Throughput Screening for Modulators of Protein-Protein Interactions: Use of Photonic Crystal Biosensors and Complementary Technologies

Heeres, J. T.; Hergenrother, P. J.

Chem. Soc. Rev. 2011, 40, 4398-4410.

 

Link to journal

Article PDF

68. 

Preparation of the Caspase-3/7 Substrate Ac-DEVD-pNA by Solution-Phase Peptide Synthesis

Peterson, Q. P.; Goode, D. R.; West, D. C.; Botham, R. C.; Hergenrother, P. J. 

Nature Protocols 20105, 294-302.

 

Link to journal

Article PDF

67. 

Identifying Modulators of Protein-Protein Interactions Using Photonic Crystal Biosensors

Heeres, J. T.; Kim, S.-H.; Leslie, B. J.; Lidstone, E. A.; Cunningham, B. T.; Hergenrother, P. J.

J. Am. Chem. Soc. 2009, 131, 18202-18203.

 

Link to journal

Article PDF

 

BioPhotonics Highlight

56.

A General Method for Discovering Inhibitors of Protein-DNA Interactions Using Photonic Crystal Biosensors

Chan, L. C.; Pineda, M.; Heeres, J. T.; Hergenrother, P. J.; Cunningham, B. T.

ACS Chem. Biol. 20083, 437-448.

 

Link to journal

Article PDF

 

About the Authors

RSC News Highlight

Biooptics World Highlight

Medical News Today Highlight

Photonics.com Highlight

55.

Identification of the Cellular Targets of Bioactive Small Organic Molecules Using Affinity Reagents 

Leslie, B. J.; Hergenrother, P. J. 

Chem. Soc. Rev. 2008, 37, 1347-1360.

 

Link to journal

Article PDF

36. 

Obtaining and screening compound collections: A user’s guide and a call to chemists

Hergenrother, P. J.  

Curr. Opin. Chem. Biol. 2006, 10, 213-218.

 

Link to journal

Article PDF

26. 

Direct Quantitation of Poly(ADP-Ribose) Polymerase (PARP) Activity as a Method to Distinguish Necrotic and Apoptotic Death in Cell and Tissue Samples

Putt, K. S.; Beilman, G. J.; Hergenrother, P. J.

ChemBioChem 20056, 53-55. 

 

Link to journal

Article PDF

22. 

A non-radiometric, high-throughput assay for poly(ADP-ribose) glycohydrolase (PARG): Application to inhibitor identification and evaluation

Putt, K. S.; Hergenrother, P. J. 

Anal. Biochem. 2004333, 256-264. 

 

Link to journal

Article PDF

21. 

An Enzymatic Assay for Poly(ADP-Ribose) Polymerase (PARP-1) via the Chemical Quantitation of NAD+: Application to the High-Throughput Screening of Small Molecules as Potential Inhibitors

Putt, K. S.; Hergenrother, P. J. 

Anal. Biochem. 2004326, 78-86. 

 

Link to journal

Article PDF

Please reload

Professor Paul J. Hergenrother

hergenro@illinois.edu

261 Roger Adams Lab

600 S. Mathews Avenue

Urbana, IL 61802

The Chemistry Department at the University of Illinois

Copyright © 2014 The Hergenrother Group

Julie Bass

Administrative Assistant

jlbass2@illinois.edu

245 Roger Adams Lab

#PJHteam